Photosynthesis Non Electron Flow and The Calvin Cycle Amusical Lecture by Glenn for joining us for Episode Two, Wher more photosynthesic waits for you. We last saw how plant cells create, O¬¬2, ATP, NADPH. Now we’ll start a twist on the light reactions, cyclic flow, we’ll see how it happens. Then we will investigate how the Calvin Cycle makes (Part Cyclic Electron Flow) – cyclic flow is also called the “Z – scheme” Put Z on its site and see what I mean. Electrons rise in energy fall and rise again. Making ATP NADPH my friends. A variation on this cyclic flow. Which makes ATP, no or O. Cyclic results from the Calvin Cycle’s needs. It uses less NADPH ATP. NADPH buildup has repercussion, of inhibiting NADP+ reduction. ‘cause NADPH clogs reductase. NADP+ from moving into place. So electrons don’t go to They’re clogged, stuck like a pus. Where can they go from Nature them a place to flow. A shunt pathway moves them the ETC. I’m talking ‘bout the in PS II tou see. These energized alectrons flow and release Which as you know is used synthesize ATP. ( Part II: Calvin Now time to learn the cycle named for Calvin. Which results in the fixation of Consider that each carbon in your bones, and hair, Was once in a CO2 in the The Calvin has three parts. Fixation of carbon how it starts. The molecules rae then reduced. ‘cause reduction the key thing that happens in part 2. 2 is the payoff as you will see. produces molecule G3P A reduced molecule with of energy. And a starting point all the molecules that cells need. Part 3 sets up conditions for the cycle’s By recreating what’s the cycle’s heart. RuBP (Ribulose Bisphosphate), a five carbon molecule that seems to ‘Cause RuBP’s at the cycle’s Then reappears at very last part. The role of RuBP at start and end. Is why this is cycle like the one named Krebs. The cycle’s inputs are know you: NADPH and ATP and CO2 Fixation of carbon is by RuBP Carboxylase Enzyme enzyme’s nickname you should know. Sounds like a maker: it’s RUBISCO. An enzyme old it was already here, Before accumulated in the air. The CO2 enters through leaf stoma, diffusing the leaf and arriving at the stroma. There RuBisCO it in its active site, and squeezes it with RuBP tight. RuBP has carbons five, it has two phosphates, on each side. When CO2 combines with RuBP it makes a six carbon you won’t see. It dissociates into two 3 – phosphoglycerates, Each with three carbons one phosphate. gets phosphorylated to bisphosohoglycerate. Phosohorylated means that receives aphosphate group from ATP. This phospharylation, followed by reduction. And you there’s an enzyme with the function. Of taking electrons and H from NADPH and adding them to bisphosphoglycerate. Take off a phosphate for – Phosphate. A starting point compounds like carbohydrates. III: Calvin Cycle, conclusion) Glyceraldehyde- 3 phosphat, known as G3P, a product cells harvest, and I hope you see, how light NADPH and ATP, are to fix CO2 and make organic energy. And to complete the cycle we must regenerate. The starting point of Calvin Cycle ribulose intermediates involved in taking G3Ps, And recombining them RuBPs. Are too much for freshman You can get the details when you yors Ph. D. But know for now that to get 3 RuBPs from 5 G3Ps cost ATPs! (Part IV: Conclusion) Now this journey ends whit emphasis, On the reaction of For over four years it’s been the food chains basis. In fossils from era you can see the traces, Of tiny cells prokaryotic form. Stromatolites that lived in waters Through photosynthesis they transformed our So aerobic life live upon it. And so evolved sentient who can analyze, How photoautotrophs can You say that photosynthesis gave birth to codiyions for cons ciousness on Planet Earth!