Photosynthesis : A - The Light Reactions Lecture by Glenn Wolkenfeld A Lecture by Glenn Wolkenfeld Boys and girls tell me you think of this, Time for a ‘bout photosynthesis Cells light to combine water carbon dioxide. Gonna see electrons going for wild ride. sugar, making sweet O¬2. why should photosynthesis matter to you? Food your table. rich atmosphere. Without photosynthesis you simple wouldn’d here. ( Part I, The Big is a redox reaction. Powered by using enzymatic action. oxidized, CO2’s re duced The sugar that results you drink in orange overall reaction has two phases. First is the light reactions which has as its powered production of ATP. O2 is the by – product and you see. of electron carrier NADPH Which provides reducing power needed in second phase ‘Cause NADPH, during phase Provides what’s needed for reducing NADPH ia by a reduction of NADP+, whose function Is to absorb electrons pick up H, Gaining energy making NADPH. Phase is the Calvin Cycle makes carbohydrate, Like sugar and starches and fibers your lunch plate. It’s how plants make food eat. Photosynthesis, so sweet! (Part II: Chloroplasts, Thylakoids, Light, Chlorophyll) In a lesf there’s mesophyllic And cells with chloroplasts key to the are almost like cells in every way, With their own and their own DNA. They even reproduce splitting into half. A clue for this organelle’s independent This has a name endosymbiosis, Developed in 60s by Lynn Margulis. Inside chloroplast is the fluid stroma. true in Tokyo. It’s true in Stroma syrrounds thylakoids. Each one a hollow space inside. The thylakoid’s membrane loaded with protein. ‘Cause it’s the light reactions’ It’s about using light for powering electrons, All about grabbing the power photons. The photons needed are are those from Which has the energy to make the world The highest frequency that can see, Is violet light wavelength 380. nanometers, sounds small, But next to gamma it’s rather tall. Red light shines 750. Isn’t the visible nifty? Photons are packets light energy, Both a wave and a particle, you Phootins get adsorbed by It’s real, it’s it ain’t no figment! Most important are green Orange carotenoids, and xanthophylls. Together they absorb light, mostly red blue. The green not absorbed it reflects back to you. In chlorophyll see the porphyrin the magnesium, which makes you want to sing, metal with valence two. You’ll those electrons rocketing through. Notice the a hydrocarbon chain. Which anchors chlorophyll into the thylakoid A molecule style, so pretty, the thylakoid it makes electricity! Part III) Englemann showed us the action Used prism to break the sun’s Light into its Shone it spirogyra, filamentous algae. The O2 algae make will stimulate Which will grow any oxygen – rich area. Bacteria it over blue and red, But hardly over green, they might as well be dead! The line of growth is a of photosynthesis’ action spectrum. Note won’t match, exactly, The absorption spectra of a or b. ‘Cause the cartenoids and xanthophylls met, light frequencies the chlorophylls can’t get. And in the thylakoid all cooperate, In using to synthesize carbohydrate. Consider in isolation, Shine some light upon it (call photoexcitation) The valence in magnessium to an excited state, it’s so fun! But once up at that level, they can fall. Back to where started, like a bouncing ball. falling alectrons, release energy, Fluorescing as red light, as you can But in thylakoids, chlorophyll’s alone, Instesd a photosystem is chlorophyll’s The parts can take photons, And use their to move electrons. The system’s antenna complex does first capture, photon anergy into electron rapture. The energized electrons bounce like ball, At the reaction center, they jump but do fall. A primary acceotor grabs them from on top, And yanks them with a force center cannot stop. To this oxidized reaction we’ll return, the details of electron flow it now is time to learn. Part IV) ATP Synthesis in Non – Cyclic Electron Flow. Non - cycle flow is main pathway. Much confustion relates the way Hootoystm precedes photosystem I. It’s a fct memorize, just get it done! PS II’s captures a photon, Generates a flow of around the world, even n Haiti, They flow to center P680. There electrons not hover, Electron acceptor them over. To the electron transport In the thylakoid This chain’s like a bucket Each molecule a similar trade. They take electrons, And use their energy for pumping From stroma to thylakoid space. Packing into that place. This makes gradient across the thylkoid membrane The protons want “out” they’ll go insane. But protons can’t permeate. The won’t allow escape. only one port through which they travel. It’s the ATP Synthase The “-ase” tells you this is enzyme. Which makes ATP all time. It has binding sites for and Pi, And channels for diffusing protons whose energy. Changes binding sites’ conformation. Fusing ADP Pi which for your information. Is thylakoids make ATP. Life’s form of energy. From this might feel familiar. is made this way in mitochondria. Chemiosomosis is the ATP is the game. ( Part V: NADPH Synthesis in – Cyclic Elecron Flow) Back to our moved by the sun. Flowing from Photosystem II System I. They get to PS I lke a used up The transport chain used up their But when hits PS I’s antenna boune to reaction center. This one’s That wavelength makes electrons PS T’s acceptor grabs them away, P700 in an oxidized way. And P700 with its oxidized blues the alectrons from PS II. Bak to electron from PS It now gose for a ride, its own fun. But powering proton pumps ain’t its It to NADP+ reductase. An plant cells count upon. To pass some and electrons. To which gets reduced. It’s how get produced. (Part VI: Oxygen Production in – Cycli Electron Flow) The by – product of these light oxygen here’s how it happens. Back PS it came to arise, That P680 oxidized To lost electrons P680 liberates. Electrons water which dissociates. one oxygen and protons two, The single will meet anoher forming O2. These protons are formed in thylakoid space. proton concentration in that place. So we see a effect of water’s oxidation. enhancement of ATP creation.