Photosynthesis part A - The Light Reactions Amusical Lecture by Glenn A music Lecture by Wolkenfeld Boys and girls tell me what you think this, Time for a ‘bout photosynthesis Cells use light to water carbon dioxide. Gonna electrons going for a wild ride. Making sugar, sweet O¬2. But why should photosynthesis matter to Food on table. rich atmosphere. Without photosynthesis simple wouldn’d be here. ( Part I, Big Picture) Photosynthesis is a redox Powered by using enzymatic action. Water’s CO2’s re duced The sugar that results you in orange juice. The overall reaction two phases. First is the light reactions which has as basis. powered production of ATP. O2 is the by – product and will see. Production of electron NADPH provides reducing power needed in the second phase ‘Cause NADPH, during 2 Provides what’s needed for CO2 NADPH ia made by reduction of NADP+, whose function to absorb electrons and pick up H, energy and making NADPH. Phase 2 is Calvin Cycle makes carbohydrate, Like sugar and starches and fibers on your plate. It’s how plants make food animals Photosynthesis, so sweet! (Part II: Chloroplasts, Thylakoids, Light, Chlorophyll) In a there’s mesophyllic tissue And cells with chloroplasts key to issue. Chloroplasts are almost cells in every way, With their own ribosomes their own DNA. They even reproduce themselves, splitting half. A clue for this independent past. This theory has name endosymbiosis, Developed in the 60s by Margulis. Inside the chloroplast is fluid stroma. true in Tokyo. It’s true Roma. Stroma syrrounds little Each one has a space inside. thylakoid’s membrane is loaded with protein. it’s the light reactions’ scene. It’s all about using light for electrons, All about grabbing the power photons. The photons needed are are those light, Which has the energy to make the world The highest frequency that can see, Is light with wavelength 380. nanometers, sounds small, But next to gamma rays it’s tall. Red light shines 750. the visible spectrum nifty? are packets of light energy, Both a wave and a you see. Phootins get adsorbed by It’s real, it’s true, it ain’t no Most important are green Orange carotenoids, and yellow Together they absorb mostly red and blue. The green is absorbed it reflects back to you. In chlorophyll see the porphyrin the magnesium, which makes you want to sing, metal with valence two. You’ll see those electrons through. Notice tail, a hydrocarbon chain. Which anchors chlorophyll into the thylakoid A molecule with so pretty, In the it makes electricity! ( III) Englemann showed us the action Used a to break the sun’s Light its frequencies. Shone on spirogyra, filamentous algae. The O2 make will stimulate bacteria. will grow in any oxygen – rich area. Bacteria loved over blue and red, But grew over green, they might as well be dead! The line of growth is a refection, of photosynthesis’ action Note this won’t exactly, The absorption spectra of chlorophyll a b. ‘Cause the cartenoids xanthophylls we’ve met, Absorb frequencies the chlorophylls can’t get. And the thylakoid they all cooperate, using light to synthesize carbohydrate. Consider chlorophyll in Shine some upon it (call it photoexcitation) valence electrons in magnessium Jump an excited state, it’s so fun! But once up at level, they can only fall. Back to they started, like a bouncing ball. falling alectrons, release energy, Fluorescing as red light, as you can But in chlorophyll’s not alone, Instesd photosystem is chlorophyll’s home. The photosystem’s parts can take And use their to move electrons. The antenna complex does the first capture, Changing anergy into electron rapture. The energized bounce like a ball, At the reaction center, they jump but do fall. A primary electron acceotor grabs them on top, And yanks them with a reaction center cannot stop. To this reaction center we’ll return, But the details of electron flow it now is time learn. ( Part IV) ATP Synthesis in Non – Electron Flow. Non cycle flow is the main pathway. Much confustion to the way Hootoystm II precedes I. a fct to memorize, just get it done! PS antenna captures a photon, Generates a of electrons. All the world, even n Haiti, They flow to reaction center There electrons not hover, acceptor passes them over. To the transport chain, the thylakoid membrane. This chain’s a bucket brigade. Each has a similar trade. They take electrons, And their energy for pumping protons. From to the thylakoid space. Packing protons into place. This makes gradient across the thylkoid membrane The protons want “out” or they’ll go But the protons can’t The membrane allow escape. There’s only one port through they travel. the ATP Synthase channel. The tells you this is an enzyme. Which makes all the time. It has binding sites for ADP and And channels for protons whose knetic energy. Changes the binding sites’ Fusing ADP with Pi which for your Is how thylakoids make Life’s key of energy. From respiration this might familiar. ATP is made this way mitochondria. is the name ATP production the game. ( Part V: NADPH Synthesis in Non – Elecron Flow) Back to our moved by the sun. Flowing from II to System I. They get to PS I lke a used battery. The transport chain used up their But when light hits I’s antenna Electrons boune to reaction This one’s That makes electrons go! PS T’s electron acceptor grabs them Leaving P700 in an oxidized And P700 with its oxidized blues accepts the from PS II. Bak to electron PS I. It now gose for a ride, its own fun. But powering pumps ain’t its fate. It travels to reductase. enzyme plant cells count upon. To pass H and electrons. To NADP+ gets reduced. It’s how get produced. (Part VI: Oxygen Production in – Cycli Electron Flow) The – product of these light reactions Is oxygen how it happens. Back PS II, it came arise, That got oxidized To replace lost electrons P680 Electrons from water which Into oxygen and protons two, The single O will meet anoher forming These protons are in the thylakoid space. proton concentration in that place. So we see a side effect of water’s Is enhancement ATP creation.