Photosynthesis part A - The Light Reactions Amusical Lecture by Wolkenfeld A Lecture by Glenn Wolkenfeld Boys and girls tell what you think of this, Time for a lesson ‘bout Cells use light to water carbon dioxide. Gonna see electrons going for wild ride. Making sugar, making sweet But why should matter to you? Food on your O2 rich Without photosynthesis you simple be here. Part I, The Big Picture) Photosynthesis a redox reaction. Powered by light using action. Water’s oxidized, CO2’s re The sugar that you drink in orange juice. overall reaction has two phases. First is the light reactions has as its basis. Light powered production ATP. O2 is the by – product and you will Production of electron carrier Which provides power needed in the second phase ‘Cause NADPH, phase 2 Provides what’s needed reducing CO2 NADPH ia made by a reduction of NADP+, function Is to absorb electrons and up H, Gaining energy and NADPH. Phase 2 is the Cycle makes carbohydrate, Like sugar and starches and on your lunch plate. It’s plants make food animals eat. it’s so sweet! (Part II: Chloroplasts, Thylakoids, and Chlorophyll) In lesf there’s mesophyllic tissue And cells with chloroplasts key to the Chloroplasts are almost like cells every way, With their own ribosomes and their own They even themselves, splitting into half. clue for this organelle’s independent past. This theory has name endosymbiosis, in the 60s by Lynn Margulis. Inside chloroplast is the fluid stroma. It’s in Tokyo. It’s true Roma. Stroma little thylakoids. one has a hollow space inside. The thylakoid’s membrane loaded with protein. ‘Cause the light reactions’ scene. It’s all about using for powering electrons, All grabbing the power in photons. The photons are are those from light, has the energy to make the world bright. The highest frequency that we see, Is violet light wavelength 380. 380 sounds small, But next gamma rays it’s rather tall. light shines at 750. Isn’t the visible spectrum Photons are packets of light Both a wave and a particle, see. Phootins get adsorbed by real, it’s true, it ain’t no figment! important are green chlorophylls. Orange carotenoids, and xanthophylls. Together they light, mostly red and blue. The green is absorbed it reflects back to you. In chlorophyll see porphyrin ring. See the magnesium, which makes you want sing, Magnesium, metal valence two. You’ll see electrons rocketing through. Notice the a hydrocarbon chain. Which anchors chlorophyll into the thylakoid A molecule with style, so the thylakoid it makes electricity! ( III) showed us the action spectrum. Used a prism to break the Light its frequencies. Shone it spirogyra, filamentous algae. The algae make will stimulate bacteria. Which will grow in any oxygen rich area. Bacteria it over blue and red, But hardly over green, they might as well be dead! The line of growth a refection, of photosynthesis’ action spectrum. this won’t match, exactly, The absorption spectra of chlorophyll or b. ‘Cause the cartenoids and xanthophylls met, light frequencies the chlorophylls can’t get. And in the thylakoid they cooperate, In light to synthesize carbohydrate. Consider chlorophyll in Shine some light upon (call it photoexcitation) The valence in magnessium to an excited state, it’s so fun! once up at that level, they can only fall. Back where they started, like a bouncing ball. falling alectrons, release energy, Fluorescing as red light, as you see. in thylakoids, chlorophyll’s not alone, a photosystem is chlorophyll’s home. The photosystem’s parts can take And use their energy to move The system’s antenna complex does the first Changing photon into electron rapture. The electrons bounce like a ball, At the reaction they jump but do not fall. A electron acceotor grabs them from on top, And them with a force reaction center cannot stop. To oxidized reaction center we’ll return, But the details of electron flow it now time to learn. ( IV) ATP Synthesis in Non – Cyclic Electron Flow. Non - cycle flow the main pathway. Much confustion relates the way Hootoystm II precedes photosystem It’s fct to memorize, just get it done! II’s antenna captures a photon, Generates a flow of All the world, even n Haiti, flow to reaction center P680. There do not hover, Electron acceptor them over. To electron transport chain, In the thylakoid chain’s like a bucket brigade. Each molecule has similar trade. They take electrons, And use energy for pumping protons. From stroma the thylakoid space. Packing protons into that This makes gradient across the thylkoid membrane The protons want or they’ll go insane. But the can’t permeate. The membrane won’t escape. only one port through which they travel. the ATP Synthase channel. “-ase” tells you this is an enzyme. makes ATP all the time. It has sites for ADP and Pi, channels for diffusing protons whose knetic energy. Changes the sites’ conformation. Fusing with Pi which for your information. Is how make ATP. key form of energy. respiration this might feel familiar. ATP made this way in mitochondria. is the name ATP production is the ( Part V: Synthesis in Non – Cyclic Elecron Flow) Back to our electons by the sun. Flowing from Photosystem to System I. They get to PS I lke used up battery. The transport used up their energy. when light hits PS I’s antenna Electrons to reaction center. This P700, That wavelength makes go! PS T’s electron acceptor grabs them Leaving P700 in oxidized way. And P700 its oxidized blues accepts the alectrons from PS II. Bak to electron PS I. It now gose for ride, having its own fun. But powering proton ain’t its fate. travels to NADP+ reductase. enzyme plant cells count upon. To pass some H and To NADP+ gets reduced. It’s NADPH get produced. (Part VI: Oxygen Production in – Cycli Electron Flow) The by – product of light reactions Is here’s how it happens. Back PS II, it came to That P680 oxidized To lost electrons P680 liberates. Electrons water which dissociates. Into one oxygen and protons The single O will meet forming O2. These protons are formed the thylakoid space. Increasing proton in that place. So see a side effect of water’s oxidation. Is enhancement ATP creation.