Photosynthesis : part A - The Reactions Amusical by Glenn Wolkenfeld A music by Glenn Wolkenfeld Boys and girls tell me what think of this, Time for lesson ‘bout photosynthesis Cells use light combine water carbon dioxide. Gonna see electrons going for a ride. Making sugar, sweet O¬2. But why should photosynthesis matter to Food on table. rich atmosphere. Without photosynthesis you simple be here. ( Part I, The Picture) Photosynthesis is a reaction. Powered by light enzymatic action. oxidized, CO2’s re duced The sugar that results you drink in juice. The overall reaction has two First is the light which has as its basis. Light production of ATP. O2 is the by – product and will see. Production of electron NADPH Which provides reducing power needed the second phase NADPH, during phase 2 Provides what’s needed for CO2 NADPH ia made by reduction of NADP+, whose function Is to absorb electrons and up H, Gaining energy and making 2 is the Calvin Cycle makes carbohydrate, Like sugar and starches and fibers on your lunch It’s how plants make food animals Photosynthesis, it’s so (Part II: Chloroplasts, Thylakoids, and Chlorophyll) a lesf there’s mesophyllic tissue And cells chloroplasts key to the issue. Chloroplasts are almost like cells every way, With their ribosomes and their own DNA. They even themselves, splitting into half. A clue this organelle’s independent past. theory has a name endosymbiosis, Developed in the 60s by Lynn Inside the chloroplast is the stroma. It’s true in true in Roma. syrrounds little thylakoids. Each one has hollow space inside. The thylakoid’s membrane is with protein. ‘Cause it’s the light scene. It’s all about using light for powering All grabbing the power in photons. The photons needed are those from light, Which has the energy to make the world The highest frequency that we can violet light with wavelength 380. 380 sounds small, But next to gamma rays rather tall. light shines at 750. Isn’t the visible nifty? Photons are of light energy, Both a wave and a you see. get adsorbed by pigments. It’s real, true, it ain’t no figment! Most important green chlorophylls. carotenoids, and yellow xanthophylls. Together they absorb light, mostly and blue. The green is not absorbed it reflects back to chlorophyll see the porphyrin ring. See the magnesium, which makes you to sing, Magnesium, with valence two. You’ll those electrons rocketing through. Notice tail, a hydrocarbon chain. Which anchors chlorophyll into the membrane. A molecule with style, so In the thylakoid it makes ( III) Englemann showed us action spectrum. Used a prism to break sun’s Light into its Shone it on filamentous algae. The O2 algae make will stimulate Which grow in any oxygen – rich area. Bacteria loved over blue and red, But hardly over green, they might as well be dead! The line of growth is a refection, of photosynthesis’ action Note this won’t match, The absorption spectra of chlorophyll or b. ‘Cause the cartenoids and xanthophylls we’ve Absorb light the chlorophylls can’t get. in the thylakoid they all cooperate, In using light synthesize carbohydrate. chlorophyll in isolation, Shine some light upon it (call it The valence in magnessium Jump to an state, it’s so fun! But once up at that they can only fall. Back to where they like a bouncing ball. These falling alectrons, release Fluorescing as red light, as you see. But thylakoids, chlorophyll’s not alone, Instesd a photosystem is chlorophyll’s The photosystem’s parts take photons, And use their energy to electrons. The system’s antenna complex does the capture, Changing photon anergy into electron The energized electrons bounce a ball, At the reaction they jump but do not fall. A primary electron acceotor grabs from on top, And yanks with a force reaction center cannot stop. To this oxidized reaction center return, But the details of flow it now is time to learn. ( Part IV) ATP Synthesis in – Cyclic Electron Flow. - cycle flow is the main pathway. Much confustion relates to the Hootoystm precedes photosystem I. It’s a fct to memorize, just get it PS II’s antenna captures a Generates a of electrons. All around the even n Haiti, They flow to center P680. There do not hover, acceptor passes them over. To the transport chain, In the thylakoid This chain’s like a bucket Each has a similar trade. They take energized And use their for pumping protons. From to the thylakoid space. Packing protons that place. makes a gradient across the thylkoid membrane protons want “out” or they’ll go insane. But the can’t permeate. The won’t allow escape. There’s one port through which they travel. It’s the Synthase channel. The “-ase” tells you this an enzyme. Which makes ATP all time. It has binding for ADP and Pi, And channels for diffusing whose knetic energy. Changes the sites’ conformation. ADP with Pi which for your information. Is how thylakoids ATP. Life’s key form of From respiration might feel familiar. ATP is made way in mitochondria. Chemiosomosis is name ATP is the game. ( Part V: Synthesis in Non – Cyclic Elecron Flow) Back to our electons moved the sun. Flowing from Photosystem II to I. They get to PS I a used up battery. The chain used up their energy. But light hits PS I’s antenna Electrons boune to reaction one’s P700, wavelength makes electrons go! PS T’s electron acceptor them away, P700 in an oxidized way. And P700 its oxidized blues accepts the alectrons from PS II. Bak electron from PS I. It now for a ride, having its own fun. But powering proton pumps ain’t its It travels to NADP+ An enzyme plant cells count pass some H and electrons. To NADP+ which reduced. how NADPH get produced. (Part VI: Oxygen Production in Non – Cycli Flow) The by – product these light reactions Is here’s how it happens. Back PS II, came to arise, That got oxidized To replace lost electrons liberates. Electrons from which dissociates. Into one oxygen and two, The single O will meet forming O2. These protons are in the thylakoid space. Increasing proton concentration in that we see a side effect of water’s oxidation. Is enhancement ATP creation.